Fig. 7.

The effect of secreted BDNF on cultured hippocampal slices. a Neutralization of BDNF diminished the number of NF200-positive neurons, abolishing the neuroprotective role of this factor. Immunostaining of hippocampal organotypic slices: neuronal marker NF200 (green), dead cell indicator PI (red), cell nuclei tracker Hoechst 33258 (blue).Scale bar =200 μm. b The measurement of BDNF concentration in control hippocampal slices (Hcontr), hippocampal slices subjected to OGD procedure (HOGD), oligodendroglial progenitors (OPC) and mature oligodendrocytes (OLS). The statistical differences of the data analyzed is p < 0.05