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The relative cleavage activities of A. thaliana OTU3, OTU4c, OTU7a, OTU9, and OTU10 for UB- and RUB-fused GST. The cleavage activities were analyzed using His-tagged OTU3, OTU7a, and OTU9 as well as His/GST-tagged OTU4c and OTU10. The substrates incubated without enzymes were used as negative input controls (Inp). The specific substrate used is indicated by “+.” The cleavage products Ub and Rub, which are labeled on the left, and the inputs were visualized by immunoblotting with α-HA.
