Table 2.
Distinct biochemical properties of A. thaliana OTU-containing deubiquitinases.
| Namea | Tag | Ub4 | Linear chain | Ub/Ubl-GST fusion | Chainb | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| K48 | K63 | Ub2 | Ub3 | Ub4 | Ub | Rub | Sumo | |||
| OTU1 | His | +++c | + | +++ | +++ | +++ | − | − | − | − |
| OTU1 | GST | +++ | + | +++ | +++ | +++ | − | − | − | − |
| OTU2 | His | + | + | − | − | − | − | − | − | K48 ≈ K63 |
| OTU3 | His | ++ | +++ | − | + | + | ++ | + | − | − |
| OTU4c | GST | +++ | +++ | − | ++ | ++ | ++ | + | − | − |
| OTU4d | His | − | − | − | − | − | − | − | − | − |
| OTU5b | His | − | − | − | − | − | − | − | − | − |
| OTLD1a | His | − | − | − | − | − | − | − | − | K48 > K63 |
| OTU7a | His | + | ++ | − | − | − | ++ | + | − | − |
| OTU9 | His | ++ | ++ | − | − | − | ++ | + | − | − |
| OTU10 | GST | ++ | +++ | − | − | − | ++ | + | − | − |
| OTU11a | His | − | − | − | − | − | − | − | − | − |
| OTU12 | His | − | − | − | − | − | − | − | − | − |
| HsOTUB1 | His | +++ | − | − | − | − | − | − | − | ND |
a
OTU4d contains a disrupted OTU domain.
b
UB chain binding was analyzed by GST pull-down assays using GST-fused proteins; –, absence of binding; ND, not determined.
c
The approximate deubiquitylation activities are quantitatively designated by +++, ++, +, and − for strong, moderate, weak, and absent activity, respectively.