Fig. 2.

Effect of PTX on the HDL-induced COX-2 expression and PGI-2 release. a–c Cells were washed and pretreated with PBS or 100 ng/ml PTX for 24 h and then stimulated without or with 120 μg/ml HDL for 8 h. The expression of COX-2 was assayed by Western blot analysis (a), relative protein expression of COX-2 was normalized to GAPDH, and the production of PGI-2 was determined by competitive ELISA (c). Data were mean ± SE from three separate experiments. *P < 0.05 versus Control group