Fig. 4.

Phosphorylation of signaling pathways involved in COX-2 expression and PGI-2 release induced by HDL in HUVECs. a HUVECs were incubated with 120 μg/ml of HDL at different time points, and the activation of PKCα and ERK1/2 was analyzed by Western blot analysis. Protein samples were immunoblotted with anti-phospho-PKCα, anti-phospho-ERK1/2, anti-total-PKCα, anti-total-ERK1/2 and anti-GAPDH antibodies