Skip to main content
. 2014 Feb 20;2014:283962. doi: 10.1155/2014/283962

Table 5.

Specificity of phenylalanine uptake in gap1 cells grown in MP and MA mediaa.

Unlabelled amino acid added Percentage inhibition
Y294Δgap1 Y294Δbap2Δgap1 Y294Δagp1Δgap1
MP MA MP MA MP MA
Leucine 39 45 34 34 4 51
Tryptophan 17 18 18 25 4 23
Asparagine 45 29 48 35 4 0
Proline ND 8 ND 13 ND 12
DL-TFLb ND 30 ND 3 ND 37
β-2TAc ND 43 ND 31 ND 55

aHigh-affinity phenylalanine uptake was measured at 4 min with L-[14C]-phenylalanine 20 μM and the unlabelled amino acids were added at 200 μM. Control values in the absence of competitor in strains Y294Δgap1, Y294Δbap2Δgap1, and Y294Δagp1Δgap1 grown in MP medium were 1.89, 1.63, and 0.44 and for cells grown in MA medium were 1.59, 0.98, and 1.09 μmol g−1, respectively.

bDL-trifluoroleucine.

c β-2-thienylalanine.