Table 5.
Unlabelled amino acid added | Percentage inhibition | |||||
---|---|---|---|---|---|---|
Y294Δgap1 | Y294Δbap2Δgap1 | Y294Δagp1Δgap1 | ||||
MP | MA | MP | MA | MP | MA | |
Leucine | 39 | 45 | 34 | 34 | 4 | 51 |
Tryptophan | 17 | 18 | 18 | 25 | 4 | 23 |
Asparagine | 45 | 29 | 48 | 35 | 4 | 0 |
Proline | ND | 8 | ND | 13 | ND | 12 |
DL-TFLb | ND | 30 | ND | 3 | ND | 37 |
β-2TAc | ND | 43 | ND | 31 | ND | 55 |
aHigh-affinity phenylalanine uptake was measured at 4 min with L-[14C]-phenylalanine 20 μM and the unlabelled amino acids were added at 200 μM. Control values in the absence of competitor in strains Y294Δgap1, Y294Δbap2Δgap1, and Y294Δagp1Δgap1 grown in MP medium were 1.89, 1.63, and 0.44 and for cells grown in MA medium were 1.59, 0.98, and 1.09 μmol g−1, respectively.
bDL-trifluoroleucine.
c β-2-thienylalanine.