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. 2014 Mar 12;4:4361. doi: 10.1038/srep04361

Figure 2. Cell-permeable OCT4, SOX2, KLF4, CMYC and NANOG induce iSC colonies (Protocol 1).

Figure 2

(A) Experimental timeline. Human dermal fibroblasts (HDF) were plated (day zero, D0), allowed to attach overnight and were treated daily with HDF media alone (no treatment), with 1 μM (each) recombinant OCT4, SOX2, KLF4, CMYC and NANOG proteins (RFs) or the same proteins containing MTD sequences to promote intracellular delivery (CP-RFs). The media was changed to hES media on day 7 (D7) and protein treatments (24 hrs/day) ended on day 16 (D16). (B) Colony morphologies. Photomicrographs of 4 representative iSC colonies induced by CP-RF treatment compared to a murine embryonic stem (mES) cell colony. (C) CP-RF induced colonies express alkaline phosphatase.