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(A) OCT4 and NANOG Immunostaining. Untreated HDFs and colonies induced by treatment with CP-RF (Protocol 2) were stained. (B–C) TRA-1-60 and SSEA-3 expression (B) and relative numbers of single and double positive cells (C). The data are presented as means ± s.d. (n = 3/No Treatment; 12/CP-RFs). * p < 0.05 and ** p < 0.01 as determined by a Student's unpaired t-test.
