Figure 2.

Comparative analysis of Pip6a-PMO uptake and exon skipping in H2k mdx skeletal muscle cells. Differentiated and non-differentiated H2k mdx cells were incubated 4 h with Pip6a-PMO at the indicated concentrations. The dose-depended internalization was assessed by fluorescence spectroscopy (A). Using the same differentiated H2K mdx samples, exon-skipping efficiency cells was evaluated by RT-PCR (B). (%ES calculated as described in Figure 1 with n ≥ 4).