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. 2013 Dec 13;42(5):2958–2975. doi: 10.1093/nar/gkt1232

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Published by Oxford University Press. 2013. This work is written by US Government employees and is in the public domain in the US.

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Figure 4. — BAF250a-containing BAF complexes regulate chromatin in early heart. (A) ChIP–qPCR showing BAF250a occupancy compared with immunoglobulin-G (IgG) in E9.5 hearts. (B) Luciferase reporter assay in P19 cells introduced with Gata4 promoter reporter construct and BAF250a siRNA. Fold changes (1.5) are shown compared with NTC (non-target control) *P < 0.001. The data are expressed as mean ± SD. (C), (D), (E), (F) Chromatin from the developing hearts was immunoprecipitated with BRG1, BAF53a, CHD4 and HDAC1 antibodies. DNA was analyzed by qPCR to determine if BRG1, BAF53a, CHD4 and HDAC1 occupy on the BAF250a binding DNA of cardiac genes. A non-genic fragment of chromosome 3 was used as negative genomic control. Data were plotted as the percentage of total input or chromatin bound. Each experiment was repeated twice.