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. 2013 Dec 9;42(5):2906–2918. doi: 10.1093/nar/gkt1267

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — T. brucei Asf1A is nuclear, whereas Asf1B is predominantly cytosolic. Panels (A) and (B) show IFA of procyclic and bloodstream T. brucei, respectively, using specific anti-Asf1A and anti-Asf1B antibodies (antibody), DAPI to stain DNA, merged and DIC images. Panel (C) shows IFA using anti-Myc monoclonal antibody, DAPI staining, merged images and DIC of wild-type procyclics (427) or procyclics expressing the endogenously 12×Myc-tagged versions of Asf1 or Asf1B. Panel (D) shows representative images of procyclics containing the plasmids pRP-Asf1A and pRP-Asf1B, which enable induced overexpression of proteins tagged with a 6×Myc peptide at the N-terminus. The analysis was performed in cells after 48 h without (−Tet) or with tetracycline-induced (+Tet) protein overexpression. Bars = 5 μm.