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. 2013 Dec 9;42(5):2906–2918. doi: 10.1093/nar/gkt1267

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 8. — Cytosolic but not the nuclear Asf1 binds to histones in vitro. (A) Bacterial extracts containing MBP-tagged Asf1c or Asf1n were incubated with purified histone H3/H4 complexes. Protein interactions were tested by pulldown assays and monitored by western blot analysis. H3/H4 input samples are shown in the lower panel. MBP was used as control. Full-length fusion proteins are marked with white asterisks (B) Interaction of MBP-tagged Asf1n and Asf1c with histone variant complexes (H3V/H4V) were analyzed as described previously. (C) Purified MBP-tagged Asf1c or Asf1n were incubated with PCF trypanosome whole cell extracts from 3 × 10⁷ cells for each pulldown. Protein interactions were analyzed as described previously.