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. 2013 Dec 11;42(5):3409–3418. doi: 10.1093/nar/gkt1279

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Unsupervised 3D classification. The 195 432 particles were first refined with RELION (27) to give a 9.1-Å ribosome reconstruction having low occupancy for the factors and tRNAs (top). The particles were then classified with RELION (27) starting with 10 seeds. The 10 resulting maps of the ribosome (bottom) are shown from the P stalk side (top vignette), with a close-up on the eRF1-eRF3 binding site (middle vignette), and from the 40S solvent side (bottom vignette). Bottom table: strength of the electron density for each domain of eRF1, eRF3 and tRNAs, as well as position of the L1 stalk.