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. 2013 Dec 11;42(5):3246–3260. doi: 10.1093/nar/gkt1281

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 8. — Growth, polysome and rRNA processing analysis for Δbmt5 and Δbmt6 mutants. (A) Ten-fold serial dilutions of the strains were spotted onto solid YPD plates and were incubated at different temperatures. (B) Polysome profile of isogenic wild type (WT), Δbmt5 and Δbmt6 mutant. (C) Illustration for the 35S primary transcript. 35S rRNA contains 18, 5.8 and 25S rRNA sequences separated by ITS1 and ITS2. The processing of the 35S precursor to mature rRNA involves endonucleolytic and exonucleolytic steps at specific sites. (D) Northern blot analysis of the Δbmt5 and Δbmt6 mutant. The membrane was hybridized with radioactive (³²P) labeled probes for ITS1, (f) for panel (i) and (e) for panel (ii), for ITS2, (i) for panel (iii) and to oligonucleotides specific to 18S (d) and 25S rRNA (j) for panel (iv). Loss of m³U methylations does not influence the growth and rRNA processing.