Figure 1.

Schematic picture of the experimental strategy. (A) met1-3 and ddm1-8 mutant plants were propagated and genotyped in each generation. Material for analyses was collected from ddm1-8−/− homozygous individuals and met1-3+/− heterozygous plants; met1-3−/− homozygous mutants were selected with extremely low frequency and did not grow to the reproductive stage (48). G0 of ddm1-8 plants represents the T3 progeny of the original accession. In the fourth generation (G4), segregated wt plants (MET1-3+/+) were selected for analysis of telomere length. (B) Arabidopsis thaliana seedlings were germinated in the presence of hypomethylation drugs DHPA or ZEB for 7 days. Plants were then cultivated in soil, and after 9 weeks, leaves were collected for analysis. Progenies (G2) of plants that had been treated with 250 µM ZEB (Z250) were grown for telomere length analysis.