Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Dec 13;42(5):3314–3329. doi: 10.1093/nar/gkt1291

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 2. — Recognition and signalling pathways following RNA transfection. (A) Effect of co-expression of BVDV N^Pro (inhibits IRF3), HCV NS3/4A (inhibits MAVS/IPS-1/CARDIF) and V-protein (inhibits MDA-5) on IFN-β induction. (B) Effect of 10 µM chloroquine (inhibits endosomal acidification and blocks TLR function) on IFN-β induction. (C) shRNA knockdown of RIG-I, MDA-5 and PKR and effect on IFN-β induction. (D) Transfection of RNAs into Huh7 and HuH7.5 (non-functional RIG-I) hepatoma cells. The 5′ppp is a 5′-phosphorylated dsRNA control for RIG-I. (E) Effect of transcript dephosphorylation on IFN-β induction. (F) PKR inhibitors 2-amino purine (2-AP) and C16 inhibit IFN-β induction; poly-I:C was used as a control. All graphs were plotted as the mean change in expression from untreated or WT cells; bars represent the mean of three biological replicates and error bars represent ± 1 SD around the mean.