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. 2013 Dec 13;42(5):3314–3329. doi: 10.1093/nar/gkt1291

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — SG induction by transfected viral RNA. (A) SGs identified by immunodetection of G3BP (red; punctuate staining) following transfection with BV RNA and sodium arsenite treatment (positive control). (B) Reproducible induction of SGs by a panel of different viral RNA transcripts (C) Co-localization of SGs immunostained for G3BP (punctuate red dots) and for transfected viral RNA sequences containing biotin-labelled UTP followed by detection with an anti-biotin-CF633 conjugate (green). Inserts show precise co-localization of SGs and viral RNA (yellow).