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. 2013 Dec 13;42(5):3104–3118. doi: 10.1093/nar/gkt1292

Figure 5.

Figure 5.

Meiotic stages of pollen-mother cells from WT and rpa1 mutants. (A–E) Wild-type, (F–J) rpa1a, (K–O) rpa1c, (P–T) rpa1a rpa1c. Stages included are zygotene (A, F, K, P), pachytene (B, G, L), a zygotene stage representing the absence of a fully synapsed pachytene stage in the rpa1a rpa1c double mutant (Q), metaphase I (C, H, M, R), anaphase I (D, I, N, S) and anaphase II (E, J, O, T). In wild-type and rpa1c, homologs are segregated in equal number at anaphase I (D, N), followed by the separation and segregation of sister chromatids at anaphase II (E, O). In rpa1a, zygotene (F) and pachytene (G) stages are similar to WT and rpa1c. However, univalents (arrows) are present at the metaphase plate (H) leading to unequal segregation of homologous chromosomes at anaphase I (I), and anaphase II (J). In rpa1a rpa1c, multiple fragmented chromosomes were present at metaphase plate (R) and at anaphase I (S) leading to abnormal anaphase II (T).