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. 2013 Dec 18;42(5):3194–3206. doi: 10.1093/nar/gkt1293

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Bfr2 and Enp2 are required for pre-rRNA processing. Total RNA was extracted from depletion strains GAL::HA-BFR2 (A) and GAL::ENP2-myc (B) grown in YPGal (0h, lane 1), and at different depletion times after the shift in YPD (lanes 2–4). RNAs were analyzed by northern hybridization using probes directed against different rRNA precursors indicated on the right. Mature 18S and 25S rRNAs were visualized by staining with GelRed™. The short and long forms of 5.8S rRNA were detected by northern hybridization.