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. 2013 Dec 18;42(5):3194–3206. doi: 10.1093/nar/gkt1293

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Sedimentation patterns on Bfr2-depletion. (A) Sedimentation profiles of Dbp4, Bfr2, Enp2 and Mpp10. Cellular extracts were prepared from undepleted (0h) and Bfr2-depleted cells (8h) and fractionated on 7–47% sucrose density gradients. Fractions 1–16 were subjected to western blot analysis using anti-myc (Enp2), anti-Dbp4, anti-HA (Bfr2) and anti-Mpp10 antibodies. The positions of 40S and 60S ribosomal subunits, 80S ribosome and polysomes are indicated. (B) Sedimentation profile of the U3 and U14 snoRNAs. Sucrose gradient fractions were prepared as in Figure 7A, except that RNAs were extracted from fractions 1–16 and subjected to northern blot analysis with radiolabeled oligonucleotides complementary to the U3 or U14 snoRNA.