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Replacement of F29, K31 or G33 with alanine prevents EF-P recognition by PoxA. WT and mutant EF-P were aminoacylated with PoxA using 14C-α-lysine. (A) Plot shows aminoacylation of WT (circle), F29A (square), K31A (rhombus) and G33A (triangle) EF-P with 14C-α-Lys. (B) After 6 h, aminoacylation samples were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Top: coomassie stained gel; bottom: phosphor image of the gel.
