Table 3.
Conservation of the amino acid sequence of the acceptor loop from EF-P
| Positiona | Organisms with PoxA |
Organisms without PoxA |
||
|---|---|---|---|---|
| Variabilityc | Amino acid identityd | Variabilityc | Amino acid identityd | |
| Glu28 | 1.198 | Usually acid (71%) | 1.993 | Rarely acid (6%) |
| Phe29b | 1.611 | Usually Phe or Tyr (79%) | 0.952 | Usually His (82%) |
| Val30 | 0.735 | Usually Val (86%) | 0.834 | Usually Val (82%) |
| Lys31b | 1.292 | Usually Lys (71%) | 0.323 | Usually Lys (94%) |
| Pro32 | 0.000 | Pro (100%) | 0.000 | Pro (100%) |
| Gly33b | 0.000 | Gly (100%) | 0.000 | Gly (100%) |
| Lys34b | 0.000 | Lys (100%) | 0.000 | Lys (100%) |
| Gly35 | 0.000 | Gly (100%) | 0.000 | Gly (100%) |
| Gln36 | 1.484 | Usually Gln (71%) | 2.190 | Never Gln (0%) |
| Ala37 | 0.000 | Ala (100%) | 0.523 | Usually Ala (88%) |
| Phe38 | 0.371 | Usually Phe (93%) | 1.277 | Usually Phe or Tyr (88%) |
| Ala39 | 2.414 | Variable | 0.834 | Usually Val (82%) |
| Arg40 | 0.735 | Usually Arg (86%) | 0.672 | Usually Arg (82%) |
aPositions are indicated according to E. coli EF-P numbering. Positions that contact PoxA are highlighted in bold letters.
bIdentity elements for PoxA aminoacylation.
cShanon entropy was calculated as an index of variability using Protein Variability Server. A value of 0 indicates no variability at all, whereas 4.322 indicates maximum variability (equal probability to find any of the 20 amino acids) (21).
dPercentage of occurrence of the amino acids in each position is in parentheses.