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Mechanical and structural parameters of the transition at the four temperatures as estimated by the multi-curve fit of r–F relations with Equation (3)
 (°C) |
 (pN) |
 (nm) |
 (nm) |
q |
|---|---|---|---|---|
| 10 | 71 ± 3 | 2.1 ± 0.2 | 3.0 ± 0.3 | 22 ± 2 |
| 15 | 69 ± 3 | 2.1 ± 0.2 | 3.1 ± 0.3 | 22 ± 2 |
| 20 | 68 ± 3 | 2.1 ± 0.1 | 3.1 ± 0.3 | 22 ± 2 |
| 25 | 66 ± 3 | 2.2 ± 0.2 | 3.1 ± 0.3 | 22 ± 2 |
The estimates of the energetic parameters per bp are ΔE: 8.2 ± 0.3 kBT, ΔS: (14.9 ± 0.5) × 10−3kBT/K, 
: 3.2 ± 0.3 kBT, β: −0.41 kBT/K.
