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. 2013 Dec 13;42(5):2999–3008. doi: 10.1093/nar/gkt1298

Figure 1.

Figure 1.

Specific activation of target gene promoters by BglJ–RcsB. Left panel: The expression level of chromosomal promoter lacZ fusions (coordinates given relative to the transcription start site) was determined in the bglJ deletion background (allele ΔyjjP-yjjQ-bglJ) (−), in isogenic bglJC strains expressing BglJ constitutively, and in isogenic bglJC ΔrcsB mutant derivatives. The factor of activation by BglJ–RcsB (fold act.) was calculated by dividing the β-galactosidase activity (β-gal. activity) determined in the bglJC strain by the activity determined in the absence of BglJ (−). Right panel: BglJ–RcsB binding sites were mutated (mutations given in Figure 2), and the expression level of the mutant promoter lacZ fusions was determined in the absence (−) and presence of BglJ (bglJC) using isogenic bglJ deletion (ΔyjjP-yjjQ-bglJ) and bglJC strains. For the seven promoters analyzed, the mutation of the binding site abrogated their activation by BglJ–RcsB. Cultures were grown in LB medium to the exponential phase and harvested at OD600 = 0.5; β-galactosidase activities are given in arbitrary units (29); n.t. is not tested.