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. 2013 Dec 25;42(5):3059–3072. doi: 10.1093/nar/gkt1323

Figure 5.

Figure 5.

In vivo binding of c-Jun/c-Fos to cellular promoters with meAP-1 sites in activated primary human B cells. After immunoprecipitation (ChIP) of chromatin from activated naïve B cells with an α-c-Jun antibody, the enrichment of the indicated promoter regions was assayed by quantitative PCR. Shown are the results obtained from three promoter regions indicated in red that contain conventional previously identified AP-1 sites (34) and three promoter regions indicated in black, which were identified in this study and are devoid of conventional AP-1 or meAP-1 sites. Both sets of promoters served as positive and negative controls, respectively. Ten promoters that were found to contain meAP-1 sites (and lack conventional AP-1 sites in proximity) were analyzed by ChIP and the results are shown in green. The PSMC1 promoter contains two separate regions with one meAP-1 site each depicted as PSMC1-1 and -2, which were analyzed separately. Details of all promoters investigated here are listed in Table 1. Means and standard deviations of three independent experiments are shown.