Table 1.
The dissociation constants (Kd) of different constructions of HpDprA toward different types of ssDNA determined by MST
| Protein | ssDNA | Dissociation constant (Kd)nM |
|---|---|---|
| Full-length HpDprA | dT35 | 2.26 ± 0.167 |
| HpDprA(5-225) | dT35 | 30.6 ± 0.998 |
| HpDprA(5-217)WT | dT35 | 31.5 ± 0.806 |
| HpDprA(5-217)H8E | dT35 | 66.3 ± 0.721 |
| HpDprA(5-217)Y11E | dT35 | 55.9 ± 1.82 |
| HpDprA(5-217)R52E | dT35 | 9950 ± 367 |
| HpDprA(5-217)Y108E | dT35 | 351 ± 6.88 |
| HpDprA(5-217)K137E | dT35 | 7220 ± 216 |
| HpDprA(5-217)F140E | dT35 | 556 ± 28 |
| HpDprA(5-217)R143E | dT35 | 5470 ± 246 |
| HpDprA(5-217)R52E/K137E | dT35 | nd |
| HpDprA(5-217)K137E/R143E | dT35 | nd |
| HpDprA(5-217)R52E/R143E | dT35 | nd |
| HpDprA(5-217) | dT6 | nd |
| HpDprA(5-217) | dT10 | nd |
| HpDprA(5-217) | dT15 | 18 400 ± 574 |
| HpDprA(5-217) | dT20 | 2230 ± 52.3 |
| HpDprA(5-217) | dA35 | 1200 ± 35.3 |
| HpDprA(5-217) | dR30 | 370 ± 8.82 |
| HpDprA(5-217) | dsDNA30 | 831 ± 8.48 |
nd, not detected.