Figure 4. c-Met signaling regulates the maintenance of LaTP.

(A) QRT-PCR documenting the expression of c-Met in both Epcam^hi LaTP and Epcam^low SynT.

(B) IF for Epcam (green) and Laminin (red) on Wt and c-Met g-KO placenta at E9.5, 10.5 and 12.5 documenting loss of Epcam^hi cells in c-Met deficient placenta after E9.5. Arrows, Epcam^hi cells adjacent to Laminin⁺ mesenchymal cells. Scale bar 100 μm.

(C) QRT-PCR analysis of gene expression in CD9⁺ trophoblast from Wt and c-Met g-KO placentas verifying down-regulation of cell cycle regulators.

(D) IF for Epcam (green), phospho Histone-H3 (PH3, red) and Laminin (blue) on Wt and c-Met g-KO placenta at E9.5, 10.5 and 12.5 documenting premature loss of proliferative LaTP in c-Met deficient placenta. Arrows, PH3⁺ Epcam^hi cells. Arrowheads, PH3⁺ Epcam⁻ cells. Scale bar 50 μm.

(E) Treatment of cultured LaTP with c-Met inhibitor documents reduced BrdU incorporation in Epcam⁺ cells. Epcam (green), BrdU (red) DAPI (blue).

(F) Treatment of cultured LaTP with c-Met inhibitor documenting no difference in cell death of Epcam⁺ cells. Epcam (green), TUNEL (red) DAPI (blue). Positive control, cells treated with DNase I.

All error bars indicate SEM (Standard error of mean).

See also Figure S4.