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. Author manuscript; available in PMC: 2014 Nov 25.
Published in final edited form as: Dev Cell. 2013 Nov 25;27(4):373–386. doi: 10.1016/j.devcel.2013.10.019

Figure 6. c-Met signaling is required to maintain Gcm1 expression in midgestation placenta.

Figure 6

(A) QRT-PCR demonstrating loss of Gcm1 expression upon treatment of cultured LaTP with c-Met inhibitor.

(B) IF documenting loss of Gcm1 in Epcam+ cells upon treatment with c-Met inhibitor. Epcam (green), Gcm1 (red) DAPI (blue)

(C) IF documenting reduced labyrinth size in Gcm1 g-KO placenta. Scale bar 100 μm. Epcam (green), Laminin (red) DAPI (blue)

(D) Phospho-Histone H3 staining documenting reduction of mitotic Epcamhi cells (arrow) in Gcm1 KO placentas. Arrowheads, PH3 negative Epcamhi cluster. Epcam (green), PH3 (red), DAPI (blue). Scale bar 100 μm.

(E) TUNEL staining indicating apoptosis is not induced in Gcm1 g-KO placenta at E10.5. Positive control for Tunel staining is shown below. Epcam (green), TUNEL (red) DAPI (blue). Scale bar 100 μm.

(F) IF showing absence of CD71+ SynT in Gcm1 g-KO placenta. Epcam (green), CD71 (red) DAPI (blue). Scale bar 100 μm.

(G) QRT-PCR and (H) IF documenting loss of Gcm1 expression by E12.5 in c-Met g-KO placentas. Epcam (green), Gcm1 (red) DAPI (blue). Scale bar 100 μm.

All error bars indicate SEM (Standard error of mean).

See also Figure S6.