Figure 3.

Small interfering RNA (siRNA)-mediated allele-specific reduction of protein expression from reporter constructs. (a) Schematic representation of the two constructs used in the experiments. (b) Representative live images of HEK293T cells at 48 hours posttransfection of a reporter construct (Col6A3TH^WT-GFP or Col6A3TH^Δ16-GFP) together with either one of the following siRNA oligos: siRNA-1, siRNA-2, or a scrambled control (siRNA-Scr). (c) Western-blot analysis of HEK293T cells in b. Cells were lysed 48 hours after cotransfections, and membrane was probed with polyclonal anti-GFP antibody. Tubulin is shown as a loading control. (d) Quantification of Western-blot GFP expression, normalized by tubulin, measured from three independent cotransfection experiments. *P ≤ 0.05, one-tailed Mann–Whitney.