Figure 4.

Reduced intracellular retention of collagen VI following small interfering RNA (siRNA) treatment in cultured congenital muscular dystrophy type Ullrich (UCMD) fibroblasts. (a) Immunofluorescence staining of collagen VI on permeabilized cells following three consecutive treatments with siRNA-1, siRNA-2, or siRNA-Scr (scrambled control), on two UCMD cell lines (UCMD1 and 3). (b) Immunofluorescence staining of collagen VI on permeabilized cells following three consecutive treatments with siRNA-2 or control siRNA-Scr, and corresponding immunoblot from these cell lysates (right panel) showing the intracellular fraction of collagen VI. Cells were washed three times before collected and lysed, to subtract the collagen secreted in the medium.