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. 2014 Jan 28;110(5):1199–1210. doi: 10.1038/bjc.2014.14

Figure 5.

Figure 5

GG-miR-221 mediates the promoting role of GC-MSCs towards gastric cancer cells. (A) Quantitative RT–PCR analysis of miR-221 levels 48 h after GC-MSCs were transfected with an miR-221 inhibitor (anti-miR-221) or NC oligonucleotides. (B) The soft agar colony formation assay of HGC-27 cells. GC-MSCs transfected with anti-miR-221 or NC were seeded at the bottom of the wells. Colonies in the top agar were photographed. Magnification, × 40. Scale bar=200 μm. *P<0.05 vs mock. (C) The transwell migration assay of HGC-27 cells treated with GC-MSCs. GC-MSCs transfected with anti-miR-221 or NC were seeded in the lower chamber, and HGC-27 cells were seeded in the upper chamber. Magnification, × 100. Scale bar=50 μm. *P<0.05 vs mock. (D) Quantitative RT–PCR analysis of miR-221 levels 48 h after HGC-27 cells were transfected with anti-miR-221 or NC. (E) HGC-27 cells transfected with anti-miR-221 were cultured in different CMs from MSCs for 48 h, and a qRT–PCR assay for miR-221 levels in HGC-27 cells was performed. *P<0.05 vs anti-miR-221 group.