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. 2014 Feb 6;110(5):1298–1306. doi: 10.1038/bjc.2014.40

Figure 5.

Figure 5

The effect of Ras inhibitors on DJ-1-promoted cell invasion. (A) Morphological change was analysed in MDA-MB-231 cells after control vector or DJ-1-Flag transfection for 48 h with or without PD98059 (a specific inhibitor of MEK, which is an immediate downstream kinase target of BRAF). (B) Cell invasion was analysed in matrigel-coated transwell. After 48 h of DJ-1-Flag transfection with or without PD98059 (a specific inhibitor of BRAF-MEK) or LY294002 (a specific inhibitor of PI3K), the bottom cells were fixed and stained with a 0.2% solution of crystal violet in 2% ethanol for light microscope image analysis. After washing, the crystal violet stain was solubilised with 10% acetic acid solution, and the cell invasion was quantitated by measuring the absorbance at 595 nm. The statistical significance was evaluated using the Student's t-test (*P<0.05, **P<0.01, ***P<0.001). These results are from three independent experiments, and each line represents s.d. (C) Snail protein expression was also evaluated under the same experimental conditions.