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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: J Am Soc Mass Spectrom. 2014 Jan 3;25(3):444–453. doi: 10.1007/s13361-013-0776-9

Fig. 1.

Fig. 1

Comparison of Lewis A, Lewis X, H1, and H2 antigens. Standard materials are: (A) Lacto-N-difucohexaose II (Lewis A), (C) CFG Te102 (Lewis X), (E) CFG Te134 (H2), and (G) Lacto-N-fucopentaose I (H1). Comparative biological materials are (B) glycosphingolipid isolated from normal human colon tissue (Lewis A), (D) O-glycan isolated from normal human colon tissue (Lewis X), and (F) fucosylated lactosamine unit from an N-glycan isolated from COLO205 cultured cells (Lewis X). In comparison to standards, (B) appears to be Lewis A, while (D) and (F) are Lewis X. The spectrum in (H) is the fucosylated lactosamine fragment from an N-glycan (composition Hex5HexNAc4dHex1) from MCF-7 cultured cells. The fragmentation pattern in (H) appears to be a composite of the H2 and Lewis X spectra, indicating a mixture of these structures.