An example of (A) water, (B) fat, (C) opposed-phase, and (D) in-phase images of the calf in a healthy subject. The benefit of Dixon imaging is that multiple image contrasts can be acquired in single scan: water, fat, in-phase, and opposed-phase images. In addition, muscle fat fraction can be calculated by drawing an ROI (arrow) on the water and fat images (fat fraction 5 signal intensity on fat image/signal intensity on fat image 1 signal intensity on water image). The fat fraction for this subject was calculated to be 5%.