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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Exerc Sport Sci Rev. 2013 Apr;41(2):107–115. doi: 10.1097/JES.0b013e3182798a95

Figure 6. MHCEmb positive fibers in SA muscles have very high rates of protein synthesis.

Figure 6

Plantaris muscles, obtained from puromycin injected mice that had been subjected to sham or synergist ablation (SA) surgeries, were frozen adjacent to one another, cross-sectioned, and then subjected to IHC-SUnSET by triple staining for puromycin (red), embryonic myosin heavy chain (MHCEmb; blue) and laminin (green). (A) Representative triple stained image of the sham and SA muscle sections. (B) Higher magnification image of a region in the SA muscle that was enriched with MHCEmb positive fibers. (C and D) Grayscale images of the puromycin signal shown in A and B, respectively. Modified from International Journal of Biochemistry and Molecular Biology, 43(9), TA Hornberger, “Mechanotransduction and the regulation of mTORC1 signaling in skeletal muscle”, pp.1267-1276, Copyright 2011, with permission from Elsevier.