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. 2014 Feb 6;55(2):381–390. doi: 10.1093/jrr/rrt123

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© The Author 2014. Published by Oxford University Press on behalf of The Japan Radiation Research Society and Japanese Society for TherapeuticRadiology and Oncology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Fig. 1. — Isolation of crypts and single crypt cells from the duodenum and jejunum of Lgr5-EGFP-IRES-CreERT2/ROSA26-tdTomato (LRT) mice. (A) Typical images of crypts and enzymatically dissociated single crypt cells. EGFP fluorescence in crypts or dissociated cells indicates expression of the Lgr5 gene. DIC is an abbreviation for differential interference contrast. Bars, 100 µm. (B) Lgr5^high cells in dissociated single crypt cells analyzed by FACS. (C) Side-scatter^high/lysozyme⁺ cells in dissociated single crypt cells analyzed by FACS. (D) CD24⁺/EpCAM⁺ cells in dissociated single crypt cells analyzed by FACS.