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. 2014 Feb 6;55(2):381–390. doi: 10.1093/jrr/rrt123

Fig. 2.

Fig. 2.

Organoid formation from single crypt cells. (A) Typical images of cells that developed into organoids. Lumen-like cystic structures started to form on Days 5–7, budding events started on Days 7–9, and robust budding was observed on Day 12. Bars, 100 µm. (B) Organoid formation from single crypt cells isolated from 4-OHT-treated LRT mice. EGFP fluorescence represents Lgr5+ stem cells, and tdTomato fluorescence indicates the progeny of Lgr5+ stem cells. Note that EGFP+ cells expand on Day 12, compared with on Days 5–9. Bars, 100 µm.