Fig. 5.

Effect of IR on the organoid-regenerating capacity of primary organoids. (A) The number of secondary organoids formed after exposure to the respective doses of IR. The primary organoids formed in the experiments shown in Fig. 3 were mechanistically dissociated and all dissociated organoids were passaged to allow secondary organoid formation. On Day 7 after passaging, the number of organoids was counted. Data represent the mean ± SEM of two independent experiments. (B) The number of secondary organoids produced from a single primary organoid. The number of secondary organoids was divided by the number of primary organoids. Data represent the mean ± SEM of two independent experiments. Statistical analysis was performed using Dunnett's multiple comparison test.