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. Author manuscript; available in PMC: 2014 Mar 12.
Published in final edited form as: J Invest Dermatol. 2014 Feb;134(2):e17. doi: 10.1038/jid.2013.521

Figure 2. Monovalent mAbs (in the form of scFv) cause blister formation typical of pemphigus foliaceus in human skin.

Figure 2

After injection of purified antidesmoglein 1 scFv, human skin specimens were cultured for 24 hours. Histology and direct immunofluorescence (IF) are shown. 3-07/1e and 3-30/3h caused superficial epidermal blisters. All scFv, except 3-094/O1808, bound to the cell surface of epidermal keratinocytes. Immunoprecipitation and indirect IF experiments suggested that 3-094/O1808 binds to the proprotein but not to the mature protein (data not shown). mAb, monoclonal antibody; scFv, single-chain variable fragment. Reprinted with permission from Ishii et al. (2008).