Table 1.
Primary antibodies
| Antibody/clone | Host species | Reactivity | Dilution | Supplier/reference |
|---|---|---|---|---|
| Gravin/AKAP12 | Rabbit pAb | m, r | 1 : 100 | Irwin H Gelman Laboratory |
| Annexin A2/C-10 | Mouse | m, r, h | 1 : 100 | Santa Cruz Biotechnology, CA, USA |
| Annexin A3 | Rabbit | Human | 1 : 100 | Sigma-Aldrich, St Louis, MO, USA |
| Aquaporin 1 | Rabbit pAb | m, r,h | 1 : 50 | Alpha Diagn Int, San Antonio, TX, USA |
| Aquaporin 2 | Rabbit pAb | r, h | 1 : 100 | Abcam, Cambridge, UK |
| Calbindin | Rabbit pAb | m, h | 1 : 50 | Abcam, Cambridge, UK |
| CD133 (AC133) | Mouse mAb | Human | 1 : 10 | Miltenyi Biotec GmbH |
| CD133/2 (293C3) | Mouse mAb | Human | 1 : 10 | Miltenyi Biotec GmbH |
| CD24 | Mouse mAb | Human | 1 : 20 | Santa Cruz Biotechnology, CA, USA |
| CD44/(156-3C11) | Mouse mAb | Human | 1 : 100 | Abcam, Cambridge, UK |
| CD44 (OX-50) | Mouse mAb | Rat | 1 : 100 | AbDSerotec, Düsseldorf, Germany |
| Claudin-1 | Rabbit pAb | m, r, h | 1 : 50 | Abcam, Cambridge, UK |
| Claudin-3 | Rabbit pAb | m, r, h | 1 : 50 | Thermo scientific, Kalamazoo, MI, USA |
| CXCR4 | Goat pAb | m, r, h | 1 : 10 | Santa Cruz Biotechnology, CA, USA |
| Integrin αV | Rabbit pAb | Human | 1 : 50 | Sigma-Aldrich, St Louis, MO, USA |
| KIM-1 | Goat pAb | Human | 1 : 100 | R&D Systems, Inc, Minneapolis, MN, USA |
| KIM-1 | Goat pAb | Rat | 1 : 200 | R&D Systems, Inc, Minneapolis, MN, USA |
| LTA-FITC | Lectin | m, r, h | 1 : 100 | Vector Laboratories, Burlingame, CA, USA |
| Megalin | Rabbit pAb | Human | 1 : 50 | Sigma-Aldrich, St Louis, MO, USA |
| Ki-67/Mib-1 | Mouse mAb | Human | 1 : 100 | Dako, Glostrup, Denmark |
| S100A6 | Rabbit pAb | Human | 1 : 50 | Sigma-Aldrich, St Louis, MO, USA |
| SSeCKS | Rabbit pAb | m, r | 1 : 100 | Irwin H Gelman Laboratory |
| Tamm–Horsfall protein | Rabbit pAb | m, r, h | 1 : 50 | Santa Cruz Biotechnology, CA, USA |
| Vimentin | Rabbit pAb | Human | 1 : 50 | Sigma-Aldrich, St Louis, MO, USA |
| Vimentin/V9 | Mouse mAb | r, h | 1 : 100 | Dako, Glostrup, Denmark |
m = mouse; r = rat; h= human; mAb = monoclonal antibody; pAb = polyclonal antibody.