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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Mol Microbiol. 2013 Oct 4;90(4):734–743. doi: 10.1111/mmi.12395

Figure 1. Mutations in the SaeRS two component system alter production of EsxA, EssB and EssD.

Figure 1

Bacterial cultures of wild type and isogenic variants of strain Newman (A) or USA300 (B) grown overnight were diluted 1:100 in fresh medium supplemented with naïve horse serum to a final concentration of 0.2% and cultures were incubated at 37°C for 2.5 h. Proteins in lyzed culture extracts were precipitated with trichloroacetic acid, separated by SDS-PAGE and detected by immunoblotting with specific antibodies (α-EsxA, α-EssB, α-EssD or α-L6 as a loading control).