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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Mol Microbiol. 2013 Oct 4;90(4):734–743. doi: 10.1111/mmi.12395

Figure 4. Expression of esxD in trans is sufficient to restore the secretion defect of the esxD mutant strain.

Figure 4

Bacterial cultures of S. aureus USA300 or esxD mutants harboring or not a complementing plasmid (pesxD) were grown to OD600nm of 1.0 and separated into cell and medium fractions. Proteins were precipitated with trichloroacetic acid, separated by SDS-PAGE and detected by immunoblotting with specific antibodies (α-EsxA, α-EsxB, α-EsxC, α-EsxD, α-EssB, α-EssD, and α-Hla or α-L6 for fractionation and loading controls).