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. Author manuscript; available in PMC: 2015 Jan 9.
Published in final edited form as: J Med Chem. 2013 Dec 24;57(1):171–190. doi: 10.1021/jm401577c

Figure 3.

Figure 3

Exposure to compounds ent-9, ent-7, ent-11, ent-10, or ent-12 modulates single-channel currents from wild-type α1β2γ2 GABAA receptors. Sample single-channel currents and the respective open and closed time histograms in the presence of 50 μM GABA (A), GABA + 20 μM ent-9 (B), GABA + 20 μM ent-7 (C), GABA + 20 μM ent-11 (D), GABA + 20 mM ent-10 (E), or GABA + 20 μM ent-12 (F). Channel openings are shown as downward deflections. In the presence of GABA alone, the open times were 0.27 ms (35%), 2.4 ms (53%), and 4.6 ms (11%), and the closed times were 0.22 ms (53%), 1.4 ms (24%), and 8.9 ms (22%). In the presence of GABA + ent-9, the open times were 0.80 ms (52%), and 30.3 ms (48%), and the closed times were 0.24 ms (67%), 1.4 ms (28%), and 14.3 ms (6%). In the presence of GABA + ent-7, the open times were 0.64 ms (39%), and 10.9 ms (61%), and the closed times were 0.27 ms (71%), 2.1 ms (24%), and 25.0 ms (5%). In the presence of GABA + ent-11, the open times were 0.30 ms (43%), 5.0 ms (24%), and 9.7 ms (33%), and the closed times were 0.43 ms (59%), 3.0 ms (24%), and 19.6 ms (17%). In the presence of GABA + ent-10, the open times were 0.48 ms (33%), 6.6 ms (21%), and 13.2 ms (46%), and the closed times were 0.38 ms (52%), 2.8 ms (31%), and 38.5 ms (16%). In the presence of GABA + ent-12, the open times were 0.29 ms (29%), 2.1 ms (23%), and 13.2 ms (48%), and the closed times were 0.25 ms (54%), 2.0 ms (24%), and 27.8 ms (22%). The summary of averaged data from multiple patches is given in Table 4.