Table 3.

Modulation of Rat Wild-Type and Mutant α₁β₂γ₂L GABA_A Receptor Function by Steroid Enantiomers ent-9 and ent-7^a

Receptor	ent-9	ent-7
α 1 β 2 γ 2L	9.2 ± 5.2**	7.8 ± 3.9**
α1(Q241L)β2γ2L	1.3 ± 0.1**, **	1.3 ± 0.3*, **
α1(S240L)β2γ2L	9.4 ± 2.9**, #	7.6 ± 3.8*, #
α1(W245L)β2γ2L	0.9 ± 0.1#, **	1.0 ± 0.1 #,**

^aThe data give the mean (±S.D.) effect of 20 μM ent-9 or ent-7 on peak currents elicited by GABA at a concentration (5 μM for wild-type; 10 μM for mutants) producing 11-17% of maximal current. Number of cells was 4-7 at each receptor-compound combination. Receptors were expressed in HEK 293 cells. Statistical analysis applies to comparison with no effect (using paired two-sample t-test) and comparison with potentiation observed at the wild-type receptor (ANOVA with Bonferroni post-hoc correction). (

^#, not significant;

^*, p<0.05;

^**, p<0.01)