Table 4.
compd | OT long (ms) | Fraction OT long | Fraction CT long |
---|---|---|---|
Controlb
(no steroid) |
6.3 ± 2.6 | 0.13 ± 0.05 | 0.29 ± 0.06 |
ent-9 | 17.7 ± 6.6*** | 0.53 ± 0.03*** | 0.06 ± 0.03*** |
ent-7 | 12.4 ± 3.4 | 0.57 ± 0.05*** | 0.05 ± 0.01*** |
ent-11 | 9.8 ± 3.0 | 0.20 ± 0.08 | 0.11 ± 0.05*** |
ent-10 | 16.1 ± 3.9* | 0.53 ± 0.06*** | 0.21 ± 0.04 |
ent-12 | 11.5 ± 3.3 | 0.49 ± 0.04*** | 0.27 ± 0.04 |
The rat wild-type α1β2γ2L GABAA receptor was activated by 50 μM GABA in the absence (control) or presence of 20 μM ent-9, ent-7, ent-11, ent-10, or ent-12. Number of patches was 4-7. The intracluster open time histograms were best-fitted to the sums of three (control, ent-11, ent-10, and ent-12) or two exponentials (ent-9 and ent-7). The intracluster closed time histograms were fitted to the sums of three exponentials. The data give the mean duration and relative frequency (fraction) of the longest-lived intracluster open time component and the relative frequency (fraction) of the longest-lived intracluster closed-time component. Previous work has shown that potentiation by steroids is mediated by changes in was conducted using ANOVA these three parameters. 16, 30 Statistical analysis was conducted using ANOVA with Bonferroni post-hoc correction. The significance level applies to comparison to control condition.
, P < 0.05;
, P < 0.001.
Non-significant changes are not marked.
The control data are from our previous publication. 49