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. 2014 Mar 12;9(3):e90836. doi: 10.1371/journal.pone.0090836

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© 2014 Zhao et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Cell growth curve measured with MTT assay indicated siRNA mediated knockdown of CHRNA3 increased the growth rate of KYSE510 cells. B. Transwell assay indicated that knockdown of CHRNA3 increased the invasion and migration of KYSE30 cells. C. Western blot analysis indicated 3 Stealth siRNA constructs effectively knocked down CHRNA3. Depletion of CHRNA3 led to a decrease of phosphorylated YAP1 and an increased of dephosphorylated YAP1, particularly a decreased S127 phosphorylation of YAP1. D. Observation of YAP1 subcellular localization with confocal fluorescence microscopy following depletion of CHRNA3. Translocation of YAP1 (green) from the cytoplasm to the nucleus was observed after siRNA mediated knockdown of CHRNA3 in KYSE510 cells for 48 h. E. Real-time PCR test indicated YAP1 targeted genes were induced by CHRNA3 knockdown in KYSE510 cell.