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. 2014 Mar 12;9(3):e90836. doi: 10.1371/journal.pone.0090836

Figure 3.

Figure 3

A. Endogenous IP test showed positive protein interactions between YAP1 and CHRNA3/CHRNB4/CHRNA5 in KYSE510 cells. B. Positive interaction between tGFP-CHRNA3 and YAP1 was detected in cells transfected with tGFP-CHRNA3. C. GST Pull assay indicated positive interaction of exogenously expressed GST-YAP1 with CHRNA3 in KYSE510 cell. D. Confocal immunofluorescence microscopy observed colocalization of YAP1 and CHRNA3 in KYSE510 cell. The KYSE510 cells were transiently expressed tGFP labeled CHRNA3 of TureClone vector (OriGene). YAP1 was labeled with red-FITC conjugated goat anti rabbit IgG. Cell nuclei were visualized with DAPI. E. endogenous IP detected positive interactions between 14-3-3 and CHRNA3/CHRNB4/CHRNA5.