Figure 4. Protein Immunoprecipitation indicated a decreased physical association between YAP1 with 14-3-3, α-catenin, β-catenin and p63 upon nicotine administration at the concentration of 80 nM for 48 h in KYSE510 cells.

B. KYSE510 cells exposed to nicotine for more than 4 days led to upregulation of total protein level of YAP1, including both the phosphorylated YAP1 and dephosphorylated YAP1 indicated by western blot analysis.