Table 1.
Acidic oligosaccharides in Holstein-Friesian colostrum1
| m/z (expt) | m/z (cal) | m/z (delta) | Oligosaccharide2
|
Retention time (min) | |||
|---|---|---|---|---|---|---|---|
| Hex | HexNAc | NeuAc | NeuGc | ||||
| 635.232 | 635.227 | 0.005 | 2 | 1 | 8.69 | ||
| 635.229 | 635.227 | 0.0023 | 2 | 1 | 21.41 | ||
| 651.224 | 651.222 | 0.0021 | 2 | 1 | 18.20 | ||
| 676.254 | 676.254 | 0 | 1 | 1 | 1 | 23.52 | |
| 676.254 | 676.254 | 0.0008 | 1 | 1 | 1 | 16.80 | |
| 676.256 | 676.254 | 0.0028 | 1 | 1 | 1 | 14.34 | |
| 692.250 | 696.249 | 0.0015 | 1 | 1 | 1 | 14.00 | |
| 797.277 | 797.280 | −0.0029 | 3 | 1 | 1 | 19.45 | |
| 797.278 | 797.280 | −0.0018 | 3 | 1 | 1 | 21.29 | |
| 838.307 | 838.307 | 0.0004 | 2 | 1 | 1 | 14.75 | |
| 838.308 | 838.307 | 0.0019 | 2 | 1 | 1 | 12.27 | |
| 1000.354 | 1000.359 | −0.0049 | 3 | 1 | 1 | 19.53 | |
| 1000.355 | 1000.359 | −0.0047 | 3 | 1 | 1 | 26.40 | |
| 1000.356 | 1000.359 | −0.0032 | 3 | 1 | 1 | 18.56 | |
| 1000.357 | 1000.359 | −0.0028 | 3 | 1 | 1 | 22.11 | |
| 1041.390 | 1041.386 | 0.0041 | 2 | 2 | 1 | 21.60 | |
| 1162.416 | 1162.412 | 0.0038 | 4 | 1 | 1 | 22.75 | |
| 1162.416 | 1162.412 | 0.0039 | 4 | 1 | 1 | 26.97 | |
| 1162.417 | 1162.412 | 0.0044 | 4 | 1 | 1 | 26.16 | |
| 1203.442 | 1203.439 | 0.0033 | 3 | 2 | 1 | 26.24 | |
| 1203.442 | 1203.439 | 0.0036 | 3 | 2 | 1 | 20.78 | |
| 1203.443 | 1203.439 | 0.0041 | 3 | 2 | 1 | 24.47 | |
| 1365.487 | 1365.492 | −0.0047 | 4 | 2 | 1 | 24.59 | |
| 1365.488 | 1365.492 | −0.0031 | 4 | 2 | 1 | 25.10 | |
1
Expt = experimental; cal = calculated; delta = difference between expt and cal masses.
2
Hex = hexose; HexNAc = N-acetylhexosamine; NeuAc = N-acetylneuraminic acid or sialic acid; NeuGc = N-glycolylneuraminic acid.