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. Author manuscript; available in PMC: 2015 Apr 1.

Published in final edited form as: J Biomed Mater Res A. 2013 Oct 17;102(4):1210–1218. doi: 10.1002/jbm.a.34779

85:15 microsphere scaffolds develop a more stable scaffold that results in enhanced bone formation over longer time periods. (A) MicroCT imaging shows that the amount of bone formed is higher in the half of the defect region that is treated with 85:15 FTY720 loaded microsphere scaffold compared to the 50:50 loaded scaffolds. Yellow outline shows the image of the original defect region at day 0, the blue outline shows the image at week 6 with the new bone formation (white arrows). (B) Change in bone volume between weeks 0–2, 0–4, 0–6, and 0–9 for the empty defect ( ), the 50:50 side of the 50:50–85:15 scaffold ( ), and the 85:15 side of the 50:50–85:15 scaffold ( ). *Statistical significance, where p<0.05. This result is reflected in the histology. (C) Untreated empty defects have minimal bone formation. (D) The side treated with 50:50 microsphere scaffold swells and loses pore volume and has less bone formation (red arrows) compared to the side treated with (E) 85:15 microsphere scaffold. All histological analysis was done 9 weeks after treatment.

Inline graphic — 85:15 microsphere scaffolds develop a more stable scaffold that results in enhanced bone formation over longer time periods. (A) MicroCT imaging shows that the amount of bone formed is higher in the half of the defect region that is treated with 85:15 FTY720 loaded microsphere scaffold compared to the 50:50 loaded scaffolds. Yellow outline shows the image of the original defect region at day 0, the blue outline shows the image at week 6 with the new bone formation (white arrows). (B) Change in bone volume between weeks 0–2, 0–4, 0–6, and 0–9 for the empty defect ( ), the 50:50 side of the 50:50–85:15 scaffold ( ), and the 85:15 side of the 50:50–85:15 scaffold ( ). *Statistical significance, where p<0.05. This result is reflected in the histology. (C) Untreated empty defects have minimal bone formation. (D) The side treated with 50:50 microsphere scaffold swells and loses pore volume and has less bone formation (red arrows) compared to the side treated with (E) 85:15 microsphere scaffold. All histological analysis was done 9 weeks after treatment.